过刊浏览

烟草愈伤组织在热胁迫过程中活性氧与抗氰呼吸变化的关系

周功克, 孔英珍, 李红玉, 文江祁, 梁厚果

兰州大学干旱农业生态国家重点实验室.甘肃,兰州 730000；兰州大学生命科学学院.甘肃,兰州 730000

摘要

烟草愈伤组织在0-14h高温(40℃)胁迫期间,其体内的O~(.)_(2)和H_(2)O_(2)迅速积累,分别在2和6h左右达到极大值。同时清除它们的酶SOD和CAT活性迅速上升,极大值的分布位置分别为2和10h左右,而胁迫期间的抗氰呼吸比未胁迫(0h)时显著地下降,但在胁迫期间抗氰呼吸于6h左右出现一峰值;用外源H_(2)O_(2)和O_(2)及清除它们的酶的抑制剂AT和DCC分别处理愈伤组织,发现抗氰呼吸明显地加强。

关键词： 烟草愈伤组织; 热胁迫; 活性氧; 抗氰呼吸

Relationship between active oxygen species and the cyanide-resistant respiration in tobacco callus under heat stress

Zhou Gongke, Kong Yingzhen, Li Hongyu, Wen Jiangqi, Liang Houguo

The State Key Laboratory of Arid Agroecology.Lanzhou 730000,Gansu；China

Abstract

These results suggested that the alternative oxidase might participate in the elimination of active oxygen species in a similar way as SOD and CAT, and that the changes in rate of the cyanide-resistant respiration were associated with those in content of the active oxygen species.

Key words: Tobacco callus;Heat stress;Active oxygen species;Cyanide-resistant respiration

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

周功克, 孔英珍, 李红玉, 文江祁, 梁厚果. 烟草愈伤组织在热胁迫过程中活性氧与抗氰呼吸变化的关系[J]. 生理学报 2000; 52 (3): .

Zhou Gongke, Kong Yingzhen, Li Hongyu, Wen Jiangqi, Liang Houguo. Relationship between active oxygen species and the cyanide-resistant respiration in tobacco callus under heat stress. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).