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NaCl胁迫对高粱根、叶鞘和叶片液泡膜ATP酶和焦磷酸酶活性的影响

王宝山, 邹琦

山东师范大学生物系.山东,济南 250014；山东农业大学生命科学院.山东,泰安 271018

摘要

NaCl胁迫初期,Na~(+)主要在根和叶鞘中积累。相应地,根和叶鞘液泡膜ATP酶和焦磷酸酶水解活性、依赖ATP和PPi的质子泵活性及Na~(+)/H~(+)逆向转运活性均明显增加,根和叶鞘的生长没有受到抑制。NaCl胁迫后期,Na~(+)开始向地上部分运输并在叶片中积累。此时,叶片液泡膜质子泵和Na~(+)/H~(+)逆向转运活性开始增加,根和叶鞘的Na/K比增加,其液泡膜ATP酶和焦磷酸酶水解活性、质子泵活性和Na~(+)/H~(+)逆向转运活性下降。相应地,根和叶鞘的生长也下降。当保温介质中Na/K比超过1时,液泡膜微囊ATP酶和焦磷酸酶活性均随Na/K比的增加而下降。表明非盐生植物液泡膜质子泵在盐胁迫的初期对Na~(+)在液泡内的积累及其耐盐性起重要作用。

关键词： 高粱; 根、叶鞘和叶片液泡膜ATP酶; 根、叶鞘和叶片液泡膜焦磷酸酶; NaCl胁迫; Na~ (+)积累

Effects of NaCl stress on the tonoplast ATPase and PPase activity in roots, sheaths and blades of sorghum seedlings

Wang Baoshan, Zou Qi

Department of Biology,Shandong Teachers^ University.Jinan 250014,Shandong

Abstract

The results indicated that the tonoplast proton pump activity of nonhalophyte plays an important role in Na~(+) accumulation in vacuoles and salt tolerance during the early period of salt stress.

Key words: Sheaths and blades;Tonoplast ATPase of the roots;Sorghum bicolor;Tonoplast PPase of the roots;NaCl stress

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引用本文：

王宝山, 邹琦. NaCl胁迫对高粱根、叶鞘和叶片液泡膜ATP酶和焦磷酸酶活性的影响[J]. 生理学报 2000; 52 (3): .

Wang Baoshan, Zou Qi. Effects of NaCl stress on the tonoplast ATPase and PPase activity in roots, sheaths and blades of sorghum seedlings. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).