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极谱法测定无氧介质中根系氧气输导

吴振斌, 贺锋, 程旺元, 成水平, Darwent M J, Armstrong B, Armstrong J

中科院水生生物研究所淡水生态与生物技术国家重点实验室.湖北,武汉 430072；中南民族学院化学系.湖北,武汉 430074；赫尔大学生命科学院

摘要

介绍了利用极谱法测定凤眼莲(Eichhornia crassipes Solm.)、菰(Zizania latifolia (Geiseb) Stapf)、石菖蒲(Acorus tartarinowii Schott)、慈菇(Sagitarria sagittifolia Linn.)、香蒲(Typha angustifolia Linn.)等多种湿生植物根系在无氧介质中氧气的对外扩散速率(ODR)。实验结果表明,对于同种植物而言,新生根较老根的氧气扩散速率快。根尖区(约0-5mm范围内)氧气扩散速率最大,氧化活力最强。愈往上愈小,根基部的氧气输出速率接近为零,氧化活力几乎可以忽略。对于根长相同的不同种植物而言,香蒲的ODR最大,凤眼莲的最小。

关键词： 极谱法; 厌氧介质; 氧气扩散速率

Studies on oxygen transportation of roots in anaerobic media with polarography

Wu Zhenbin, He Feng, Cheng Wangyuan, Cheng Shuiping, Darwent M J, Armstrong B, Armstrong J

State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences.Wuhan 430072,Hubei；China；College of Life Sciences,University of Hull.UK

Abstract

The result indicated that the apical regions (about 0-5 mm from the tip) have the highest ODR and the most active oxidising activities. ODR decreased from the apical region to the base roots, near the base, ODR was almost negligible. For the same species, oxygen diffusion rate of new roots was higher than that of old olnes. For the roots of different species of the same length, ODR of Typha angustifolia Linn. was the highest, Eichhornia crassipes Solm. was the lowest.

Key words: Polarography;Anaerobic media;Oxygen diffusion rate (ODR)

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引用本文：

吴振斌, 贺锋, 程旺元, 成水平, Darwent M J, Armstrong B, Armstrong J. 极谱法测定无氧介质中根系氧气输导[J]. 生理学报 2000; 52 (3): .

Wu Zhenbin, He Feng, Cheng Wangyuan, Cheng Shuiping, Darwent M J, Armstrong B, Armstrong J. Studies on oxygen transportation of roots in anaerobic media with polarography. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).