过刊浏览

转WMV-2 CP基因黄瓜植株的再生

王慧中, 赵培洁, 周晓云

杭州师范学院生物系.浙江,杭州 310012；浙江大学农业与生物技术学院.浙江,杭州 310029；浙江工业大学生物工程系.浙江,杭州 310014

摘要

以黄瓜无菌苗子叶切段为外植体,通过叶盘转化法与根瘤农杆菌进行共培养建立了黄瓜的转基因系统。农杆菌菌株为LBA4404,内含双元载体pBPMWMV。该质粒载体带有一个npt-Ⅱ基因(筛选具有卡那霉素抗性的植株)和一个WMV-2 CP基因。抗卡那霉素(Kan~(r))的黄瓜植株经DNA分子点杂交、PCR检测以及Southern blot证实,外源的WMV-2 CP基因确实已导入黄瓜细胞且能稳定地遗传到子一代。对WMV-2 CP基因在子一代的分离进行了统计。获得的转基因子一代植株对WMV-2表现出较强的抗性,可以延迟发病时间,减轻发病程度。

关键词： 黄瓜; 子叶; 根瘤农杆菌; 外壳蛋白基因; 转基因植株; 病毒抗性

Regeneration of transgenic cucumber plants with a WMV-2 CP gene

Wang Huizhong, Zhao Peijie, Zhou Xiaoyun

Department of Biology,Hangzhou Normal College.Hangzhou 310036,Zhejiang；China

Abstract

Cotyledon segments of Cucumis staivus L. were used as explants for transformation mediated by Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pBPMWMV containing an npt-II gene and a WMV-2 CP gene. Transgenic plants were obtained and segregation of WMV-2 CP gene in R1 transgenic plants was analysed. Successful genetic transformation was confirmed by DNA dot hybridization, PCR assay and Southern blot analysis. The incidence of virus disease was low and late on transgenic plants, thus showing that it had a certain resistance to relevant viruses.

Key words: Cucumis sativus L.;Cotyledon;Agrobacterium tumefaciens;CP gene;Transgenic plant;Resistance to virus

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

王慧中, 赵培洁, 周晓云. 转WMV-2 CP基因黄瓜植株的再生[J]. 生理学报 2000; 52 (3): .

Wang Huizhong, Zhao Peijie, Zhou Xiaoyun. Regeneration of transgenic cucumber plants with a WMV-2 CP gene. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).