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东北红豆杉愈伤组织细胞分裂及染色体稳定性

王淑豪, 齐怀丰, 元英进

天津大学化工学院生物化工系.天津 300072

摘要

研究了继代4年的东北红豆杉愈伤组织细胞分裂过程,并对其染色体数目的稳定性进行了讨论及染色体核型进行了分析。取转接3-4d的东北红豆杉愈伤组织细胞,制作染色体压片,可观察到细胞有丝分裂前、中、后、末期的染色体形态;未观察到无丝分裂过程。继代4年的愈伤组织细胞的染色体倍性十分稳定,体细胞染色体数目及核型2n=24,这一结果与实生根压片所得结果一致。

关键词： 东北红豆杉; 愈伤组织; 细胞分裂; 染色体数目; 核型

Study on mitosis and chromosome stability of callus of Taxus cuspidata

Wang Shuhao, Qi Huaifeng, Yuan Yingjin

Department of Biochemical Engineering,College of Chemical Engineering,Tianjin University.Tianjin 300072

Abstract

Cell division and chromosome number stability of callus of Taxus cuspidata after subculture for 4 years have been studied. Chromosome morphology during mitosis after 3-4 d of culture have been observed. Amitosis did not occur. Chromosome counting showed that the chromosome ploidy of callus cells of T. cuspidata was very stable during subculture for 4 years. The karyotype formula is 2n=24, which agrees with that from root-tip squash of seedlings.

Key words: Taxus cuspidata;Callus;Cell division;Chromosome number;Karyotype

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引用本文：

王淑豪, 齐怀丰, 元英进. 东北红豆杉愈伤组织细胞分裂及染色体稳定性[J]. 生理学报 2000; 52 (3): .

Wang Shuhao, Qi Huaifeng, Yuan Yingjin. Study on mitosis and chromosome stability of callus of Taxus cuspidata. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).