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茉莉酸甲酯诱导烟草幼苗抗炭疽病与PAL活性及细胞壁物质的关系

宾金华, 姜胜, 黄胜琴, 潘瑞炽

华南师范大学生物系.广东,广州 610631

摘要

探讨了茉莉酸甲酯(methyl jasmonate,Me-Ja)诱导烟草幼苗抗炭疽病与苯丙氨酸解氨酶(PAL)活性、木质素和富含羟脯氨酸蛋白(HRGP)含量的关系。Me-Ja处理烟草幼苗不仅可以提高幼苗抗炭疽病的能力,而且明显提高幼苗的PAL活性、木质素和HRGP含量;3个不同抗性品种的烟草幼苗的PAL酶活性、木质素和HRGP含量三者与感病程度之间的负相关都达到显著水平,表明PAL、木质素和富含羟脯氨酸蛋白(HRGP)在茉莉酸甲酯诱导抗病中起关键作用。

关键词： 烟草幼苗; 茉莉酸甲酯; 诱导抗病性; 苯丙氨酸解氨酶; 富含羟脯氨基酸蛋白; 木质素

The relationship between methyl jasmonate induced anthracnose resistance of tobacco seedlings and phenylalanine ammonia-lyase activity and cell wall substances

Bin Jinhua, Jiang Sheng, Huang Shengqin, Pan Ruichi

Department of Biology,South China Normal University.Guangzhou 510631,Guangdong

Abstract

Three varieties of tobacco (Nicotiana tabacum L.) seedlings at 4-leaf stage were inoculated with spores (10~(8)/ml) of Colletotrichum destructivum O^Gara after or before treatment with methyl jasmonate (Me-Ja). Phenylalanine ammonia-lyase (PAL) activity, hydroxyproline-rich glycoprotein (HRGP) and lignin contents were determined on the following days. Me-Ja treament not only increased the seedlings^ resistance to anthracnose, but also significantly increased their PAL activity, HRGP contents and lignin. The resistance to anthracnose of three tobacco varieties was found to be significantly negatively correlated with the three parameters studied. It is considered that the PAL, lignin and HRGP are involved in the induction of disease resistance by Me-Ja.

Key words: Tobacco seedling;Methyl jasmonate;Induced resistance;PAL;HRGP;Lignin

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引用本文：

宾金华, 姜胜, 黄胜琴, 潘瑞炽. 茉莉酸甲酯诱导烟草幼苗抗炭疽病与PAL活性及细胞壁物质的关系[J]. 生理学报 2000; 52 (1): .

Bin Jinhua, Jiang Sheng, Huang Shengqin, Pan Ruichi. The relationship between methyl jasmonate induced anthracnose resistance of tobacco seedlings and phenylalanine ammonia-lyase activity and cell wall substances. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).