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肝细胞生长因子对四氯化碳损伤肝细胞的保护作用及相关基因表达

邹原, 宫德正, 梅懋华, 张万琴

大连医科大学生理教研室.辽宁,大连 116027

摘要

利用无血清原代培养大鼠肝细胞,观察重组人肝细胞生长因子(rhHGF)对CCl_(4)染毒肝细胞的保护作用。结果提示,rhHGF对CCl_(4)染毒大鼠肝细胞具有保护作用;EGF和rhHGF有协同保护作用;HGF及其受体的表达在肝脏再生及修复中可能有重要作用。

关键词： 重组人肝细胞生长因子; 表皮生长因子; c-met基因; 四氯化碳; 肝细胞原代培养

Protective effect of hepatocyte growth factor on hepatocyte poisoning by carbon tetrachloride and related gene expression in rats

Zou Yuan, Gong Dezheng, Mei Maohua, Zhang Wanqin

Department of Physiology,Dalian Medical University.Dalian 116027,Liaoning

Abstract

The results showed that rhHGF could protect hepatocytes against CCl_(4) through preventing intracellular ALT and potassium ion leakage, thus suggesting a synergistic effect of rhHGF and EGF on cytoprotection, and high expression of HGF and its receptor/c-met mRNA in the liver treated with PH and CCl_(4) poisoning. The present study suggests that the expression of HGF and its receptor gene plays an important role in liver regeneration and repair.

Key words: Recombinant human hepatocyte;Growth factor epidermal growth factor;c-met carbon;Tetrachloride primary;Hepatocyte culture

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引用本文：

邹原, 宫德正, 梅懋华, 张万琴. 肝细胞生长因子对四氯化碳损伤肝细胞的保护作用及相关基因表达[J]. 生理学报 2000; 52 (1): .

Zou Yuan, Gong Dezheng, Mei Maohua, Zhang Wanqin. Protective effect of hepatocyte growth factor on hepatocyte poisoning by carbon tetrachloride and related gene expression in rats. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).