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铝和铝+钙对小麦幼苗根尖质膜、液泡膜微囊ATP酶和膜流动性的影响

张芬琴, 沈振国, 刘友良

南京农业大学农业部作物生长调控重点开放实验室.江苏,南京 210095

摘要

研究了铝和铝+钙对小麦幼苗根尖质膜、液泡膜微囊H~(+)-ATP酶、Ca~(2+)-ATP酶、Mg~(2+)-ATP酶活性及其动力学参数和膜流动性的影响。在质膜和液泡膜微囊制剂中加入1.0mmol/L的Al~(3+)(AlCl_(3))时,H~(+)-ATP酶、Ca~(2+)-ATP酶、Mg~(2+)-ATP酶活性和酶促反应的V_(max)及膜流动性下降,而酶促反应的最适pH和K_(m)均不受影响。提高酶促反应介质的Ca~(2+)(CaCl_(2))浓度可以缓解Al~(3+)对膜ATP酶活性和膜流动性的影响。推测Al~(3+)可能通过与膜的结合而抑制膜ATP酶的活性。

关键词： 铝; 钙; 小麦幼苗; 质膜; 液泡膜; ATP酶; 膜流动性

Effects of Al~(3+) and Al~(3+)+Ca~(2+) on ATPases and membrane fluidities of plasma membrane and tonoplast vesicles from root tips of wheat seedlings

Zhang Fenqin, Shen Zhenguo, Liu Youliang

Key Laboratory of Crop Growth Regulation,The Ministry of Agriculture,Nanjing Agricultural University.Nanjing 210095,Jiangsu

Abstract

These results suggest that the binding of Al to the membrane results in the inhibition of the ATPase activity.

Key words: Al~(3+);Ca~(2+);Wheat seedling;Plasma membrane;Tonoplast;ATPase;Membrane fluidity

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引用本文：

张芬琴, 沈振国, 刘友良. 铝和铝+钙对小麦幼苗根尖质膜、液泡膜微囊ATP酶和膜流动性的影响[J]. 生理学报 2000; 52 (2): .

Zhang Fenqin, Shen Zhenguo, Liu Youliang. Effects of Al~(3+) and Al~(3+)+Ca~(2+) on ATPases and membrane fluidities of plasma membrane and tonoplast vesicles from root tips of wheat seedlings. Acta Physiol Sin 2000; 52 (2): (in Chinese with English abstract).