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赤霉素和光对拟南芥种子萌发和幼苗生长的影响

李凤玲, 陈季楚, 赵毓橘

中科院上海植物生理研究所.上海 200032

摘要

以拟南芥的赤霉素(GA)缺陷型突变体ga-1,ga-2,ga-3和GA不敏感型突变体ga-i为材料,研究了光和4种GA对拟南芥种子萌发和幼苗生长影响的相互关系。结果表明:(1)烯效唑对ga-i种子萌发的抑制在光下可明显被GA恢复,而在黑暗中GA的作用不明显。(2)在光下低浓度的外源GA_(3)可使ga-1,ga-2和ga-3的种子萌发,而在黑暗中同样浓度的GA_(3)则难以使种子萌发。(3)光可以降低种子萌发所需求的GA的剂量。(4)ga-i和ga-1的幼苗的呼吸代谢有明显差异。以上结果说明:光对拟南芥种子萌发的促进主要是提高种子对GA反应的敏感性而不是增加GA的生物合成。

关键词： 赤霉素; 突变体; 拟南芥

Effects of gibberellin and light on seed germination and seedling growth of Arabidopsis

Li Fengling, Chen Jichu, Zhao Yuju

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

ga-1, ga-2, ga-3 are gibberellin deficient Arabidopsis mutants, ga-i is a gibberellin insensitive mutant. The relationship between the role of gibberellin and light during seed germination and seedlings was investigated. Inhibition of ga-i germination by uniconazole and prohexadione was recovered partially by application of GA_(3) in light, but not in the dark. GA_(3) was active inducing ga-1, ga-2 and ga-3 germination in light, but not in the dark. Light lowered GA concentration required for germination from 10~(-4)mol/L in the dark to 10~(-6)mol/L in light. The respiratory metabolism of ga-1 was different from ga-i and wild type (Ler). These results show that light might increased sensitivity of Arabidopsis seed to exogenously applied GA rather than the capacity to synthesize GAs.

Key words: Gibberellins;Mutants;Arabidopsis

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引用本文：

李凤玲, 陈季楚, 赵毓橘. 赤霉素和光对拟南芥种子萌发和幼苗生长的影响[J]. 生理学报 2000; 52 (2): .

Li Fengling, Chen Jichu, Zhao Yuju. Effects of gibberellin and light on seed germination and seedling growth of Arabidopsis. Acta Physiol Sin 2000; 52 (2): (in Chinese with English abstract).