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重组人白细胞介素-1#beta#对小鼠骨髓基质细胞K~(+)通道的影响

陈娜娜, 吴曙光

第一军医大学药物研究所.广东,广州 510515

摘要

用细胞贴附式和内面向外式的膜片箝单通道记录方式研究了小鼠骨髓基质细胞K~(+)通道的动力学特性以及重组人白细胞介素-1#beta#(IL-1#beta#)对通道的影响,发现了骨髓基质细胞膜上存在一类性质类似于延迟整流的K~(+)通道的电压依赖性K~(+)通道,单通道电导为16.7±1.4pS,通道的动力学特性具显著的电压依赖性。

关键词： 白细胞介素-1; 骨髓基质细胞; K~(+)通道; 膜片箝; 信号转导

Effect of recombinant human interleukin-1#beta# on K~(+) channels of mice bone marrow stromal cells

Chen Nana, Wu Shuguang

Institute of Pharmaceutical Sciences,First Military Medical University.Guangzhou 510515,Guangdong

Abstract

In the present study, both cell-attached and inside-out mode of patch clamp technique were applied to detect the effect of recombinant human interleukin-1#beta# (IL-1#beta#) on K~(+) channels of mice bone marrow stromal cells. A 16.7 pS voltage-dependent K~(+) channel resembling the delayed rectifier K~(+) channel in excitable cells was identified and characterized.

Key words: Interleukin-1;Bone marrow stromal cell;K~(+) channels;Patch clamp;Signal transduction

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引用本文：

陈娜娜, 吴曙光. 重组人白细胞介素-1#beta#对小鼠骨髓基质细胞K~(+)通道的影响[J]. 生理学报 1999; 51 (6): .

Chen Nana, Wu Shuguang. Effect of recombinant human interleukin-1#beta# on K~(+) channels of mice bone marrow stromal cells. Acta Physiol Sin 1999; 51 (6): (in Chinese with English abstract).