过刊浏览

小鼠骨髓内皮细胞无血清条件培养液及其越滤组分对粒巨噬系祖细胞生长的影响

汪保和, 王绮如

湖南医科大学血液生理研究室.湖南,长沙 410078

摘要

关键词： 骨髓内皮细胞; 条件培养液; 生长因子; 生长抑制因子; 粒巨噬系祖细胞

Effects of bone marrow endothelial cell conditioned medium and its ultrafiltration-prepared components on the growth of CFU-GM

Wang Baohe, Wang Qiru

Laboratory of Blood Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

Assays of granulocyte-macrophage colony-forming cell (CFU-GM) were performed to examine the effects of mBMEC-CM and its ultrafiltration-prepared components. It was not observed that mBMEC-CM and MW 3-10kD component had any significant influence on the growth of CFU-GM. However, MW>10 kD and 0.5-1 kD components enhanced the proliferation of CFU-GM, whereas MW 1-3 kD and <0.5 kD ones inhibited it. All these four components exerted their effects on the growth of CFU-GM in dose-dependent way.

Key words: Bone marrow endothelial cell;Conditioned medium;Growth factor;Growth inhibitor;CFU-GM

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

汪保和, 王绮如. 小鼠骨髓内皮细胞无血清条件培养液及其越滤组分对粒巨噬系祖细胞生长的影响[J]. 生理学报 1999; 51 (6): .

Wang Baohe, Wang Qiru. Effects of bone marrow endothelial cell conditioned medium and its ultrafiltration-prepared components on the growth of CFU-GM. Acta Physiol Sin 1999; 51 (6): (in Chinese with English abstract).