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M型受体在大鼠肾上腺嗜铬细胞内钙库释放和激素分泌中的作用

徐建华, 曾旭辉, 何立铭, 瞿安连, 周专

华中理工大学生物物理与生物化学研究所.湖北,武汉 430074

摘要

激发嗜铬细胞毒蕈碱受体可引起钙库释放,并触发分泌。在无外钙条件下,还研究了连续刺激引起的[Ca~(2+)]_(i)变化,发现第一次的1mmol/L MCh刺激可升高[Ca~(2+)]_(i),但与细胞外液中含Ca~(2+)时不同的是,细胞对随后的MCh刺激不再响应。

关键词： 嗜铬细胞; M型受体; 钙离子库; 分泌; 碳纤电极

Role of M-type receptor in internal calcium release and quantal secretion in rat adrenal chromaffin cells

Xu Jianhua, Zeng Xuhui, He Liming, Qu Anlian, Zhou Zhuan

Institute of Biophysical Biochem,Huazhong University of Science and Technology.Wuhan 430074,Hubei

Abstract

In rat single adrenal chromaffin cells, the effects of methacholine (MCh) on [Ca~(2+)]_(i) and catecholamine secretion were studied with fura-2 fluorescence and carbon fiber electrodes. In the presence of 2 mmol/L Ca~(2+) in the bath, locally applying 1 mmol/L MCh, either containing or not containing Ca~(2+), evoked both [Ca~(2+)]_(i) and secretion signals. In the absence of Ca~(2+) in the bath, MCh could still evoke [Ca~(2+)]_(i) and secretion.

Key words: Chromaffin cell;mACh-receptor;Ca store;Secretion;Carbon fiber electrode

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引用本文：

徐建华, 曾旭辉, 何立铭, 瞿安连, 周专. M型受体在大鼠肾上腺嗜铬细胞内钙库释放和激素分泌中的作用[J]. 生理学报 1999; 51 (5): .

Xu Jianhua, Zeng Xuhui, He Liming, Qu Anlian, Zhou Zhuan. Role of M-type receptor in internal calcium release and quantal secretion in rat adrenal chromaffin cells. Acta Physiol Sin 1999; 51 (5): (in Chinese with English abstract).