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NaCl胁迫对小麦抗盐突变体根液泡膜H~(+)-ATP酶和H~(+)-PP酶活性的影响

郭房庆, 黄昊, 汤章城

中科院上海植物生理研究所.上海 200032

摘要

随着盐胁迫强度(NaCl 0-150mmol/L)和时间(0-72h)的增加,小麦抗盐突变体根液泡膜H~(+)-ATP酶和H~(+)-PP酶活性显著增加,虽然野生型酶活性在盐胁迫下也有增加,但其增加的幅度显著低于突变体,H~(+)-PP酶活性的差异更为显著。H~(+)-ATP酶和H~(+)-PP酶的最适pH值在两者之间以及盐胁迫前后均无改变,分别为7.0和8.0。无盐胁迫下野生型液泡膜58kD蛋白带缺失,盐胁迫下这一蛋白有微弱的表达。与此不同,突变体58kD蛋白在盐胁迫前后均有明显的表达,但其29kD蛋白带在无盐胁迫下明显减弱。

关键词： 小麦; NaCl胁迫; 液泡膜; H~(+)-ATP酶; H~(+)-PP酶

Effects of NaCl stress on H~(+)-ATPase and H~(+)-PPase activities of tonoplast-enriched vesicles isolated from the roots of salt-tolerant mutant of wheat and its wild type

Guo Fangqing, Huang Hao, Tang Zhangcheng

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The analyzing results of characterization of tonoplast polypeptides showed that the 58 kD peptide was absent in wild type under control condition and salt stress a small amount of this peptide was found. The mutant had a relatively mire amount of the 58 kD peptide under control condition or salt stress treatment, but its 29 kD peptide disappeared under control condition. We discussed the relationship between the enhancement of H~(+)-translocating abilities and the increase of H~(+)-ATPase and H~(+)-PPase activities in the tonoplast-enriched vesicles isolated from roots of the mutant and wild type treated with salt stress.

Key words: Wheat;NaCl stress;Tonoplast;H~(+)-ATPase;H~(+)-PPase

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引用本文：

郭房庆, 黄昊, 汤章城. NaCl胁迫对小麦抗盐突变体根液泡膜H~(+)-ATP酶和H~(+)-PP酶活性的影响[J]. 生理学报 1999; 51 (4): .

Guo Fangqing, Huang Hao, Tang Zhangcheng. Effects of NaCl stress on H~(+)-ATPase and H~(+)-PPase activities of tonoplast-enriched vesicles isolated from the roots of salt-tolerant mutant of wheat and its wild type. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).