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IAA对小麦胚芽鞘质膜蛋白磷酸化的影响

吴海明, 王宁宁, 于自然, 朱亮基

南开大学生物化学与分子生物学系.天津 300071

摘要

磷酸化/脱磷酸化机制是众多信号转导过程中重要环节,很多信号物质被细胞受体识别后引发蛋白激酶和蛋白磷酸酶活性变化,通过磷酸化/脱磷酸化进一步调节多种酶活性而产生各种生理效应。在对生长素IAA的信号转导的研究中,发现IAA处理的小麦胚芽鞘质膜蛋白中蛋白激酶的活性和蛋白磷酸化程度都发生改变,并找到两种受到调节的蛋白激酶。钙离子通道抑制剂LaCl_(3)阻断了IAA的这种作用,表明Ca~(2+)参与了IAA的信号转导过程。

关键词： IAA; 小麦胚芽鞘; 蛋白磷酸化; 蛋白激酶; 钙离子通道

The effect of IAA on phosphorylation of plasma proteins in wheat coleoptiles

Wu Haiming, Wang Ningning, Yu Ziran, Zhu Liangji

Biochemistry and Molecular Biology Department of Nankai University.Tianjing 300071

Abstract

Protein kinases and protein phosphatases play key roles in signal transduction. Many factors regulate enzyme activities by changing the phosphorylation level of them. Protein kinase activity was observed in plasma membrane of wheat coleoptiles. Activity of this kinase is Ca~(2+)-dependent. Treating with IAA increased the kinase activity. The phosphorylation of proteins with molecular weight 132, 100, 87, 72, 50 and 35 kD was enhanced. IAA also enhanced the autophosphorylation of protein kinases with MW 125 and 89 kD. LaCl_(3), as an inhibitor of calcium ion channel, diminished of calcium ion channel, diminished the influences of IAA. It seems possible that Ca~(2+)-dependent protein kinase functions in calcium-regulated signal transduction processes in IAA-induced elongation of wheat coleoptiles through their responses to changes in concentration of cytosolic calcium.

Key words: IAA;Wheat coleoptiles;Protein phosphorylation;Protein kinase;Ca~(2+) ion channel

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引用本文：

吴海明, 王宁宁, 于自然, 朱亮基. IAA对小麦胚芽鞘质膜蛋白磷酸化的影响[J]. 生理学报 1999; 51 (4): .

Wu Haiming, Wang Ningning, Yu Ziran, Zhu Liangji. The effect of IAA on phosphorylation of plasma proteins in wheat coleoptiles. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).