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铝胁迫对小麦根系液泡膜ATP酶、焦磷酸酶活性和膜脂组成的效应

何龙飞, 沈振国, 刘友良

南京农业大学农学系,农业部作物生长调控重点开放实验室.江苏,南京 210095

摘要

与对照相比,经20和100#mu#mol/L的AlCl_(3)处理后,耐铝品种Altas 66的液泡膜H~(+)-ATP酶和Ca~(2+)-ATP酶活性迅速下降;铝敏感品种Scout 66液泡膜H~(+)-ATP酶和Ca~(2+)-ATP酶活性则在20#mu#mol/L时增加,100#mu#mol/L时下降。焦磷酸酶活性在Altas 66中下降,在Scout 66中增加。与对照相比,在AlCl_(3) 20#mu#mol/L处理时,液泡膜磷脂含量增加,Altas 66中的增加比Scout 66更为明显;100#mu#mol/L时,Scout 66液泡膜磷脂含量迅速下降,而Altas 66下降不显著。两品种小麦在铝处理后根液泡膜糖脂结合半乳糖含量均高于对照,而Altas 66中的含量又高于Scout 66。铝处理后,两品种小麦根液泡膜的棕榈酸和油酸含量增加,亚麻酸含量下降,不饱和指数也随之下降,其中Scout 66下降更为明显。

关键词： 铝胁迫; 液泡膜; H~(+)-ATP酶; Ca~(2+); ATP酶; 焦磷酸酶; 膜脂

The responses of ATPase and PPase activities and lipid composition of tonoplast in roots of two wheat cultivars to aluminium stress

He Longfei, Shen Zhenguo, Liu Youliang

Department of Agronomy,Nanjing Agricultural University,Key Laboratory of Crop Growth Regulation,Ministry of Agriculture.Nanjing 210095,Jiangsu

Abstract

In comparison with the controls, the activities of H~(+)-ATPase and Ca~(2+)-ATPase of tonoplast vesicles in Altas 66 were decreased greatly by AlCl_(3) 20 and 100 #mu#mol/L treatment, those of Scout 66 were increased at AlCl_(3) 20 #mu#mol/L, but it decreased at AlCl_(3) 100 #mu#mol/L. The H~(+)-PPase activity of Altas 66 was decreased by Al treatment, while that of Scout 66 increased. The phospholipid contents of tonoplast vesicles in Altas 66 and Scout 66 were increased by AlCl_(3) 20 #mu#mol/L treatment, but it was decreased by AlCl_(3) 100 #mu#mol/L. The contents of galactose in lipids of two cultivars after Al treatments was higher than those of the controls and it was higher in Altas 66 than in Scout 66. After Al treatment, the palmitic acid and stearic acid contents increased, the linolenic acid content and the unsaturation index of fatty acids decreased in both cultivars, but those in Scout 66 more distinctly.

Key words: Al stress;Tonoplast;H~(+)-ATPase;Ca~(2+)-ATPase;H~(+)-PPase;Lipid composition;

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引用本文：

何龙飞, 沈振国, 刘友良. 铝胁迫对小麦根系液泡膜ATP酶、焦磷酸酶活性和膜脂组成的效应[J]. 生理学报 1999; 51 (4): .

He Longfei, Shen Zhenguo, Liu Youliang. The responses of ATPase and PPase activities and lipid composition of tonoplast in roots of two wheat cultivars to aluminium stress. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).