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羟自由基诱导烟草细胞凋亡

夏慧莉, 陈浩明, 吴逸, 戴尧仁

清华大学生物科学与技术系.北京 100084

摘要

用适当浓度组合的硫酸亚铁和过氧化氢反应以产生羟自由基来处理烟草悬浮细胞,首次观察到了细胞中染色质凝缩、边缘化、细胞核解体等典型的细胞凋亡的形态学特征;在DNA琼脂糖凝胶电泳图谱上观察到因核DNA有规则地在核小体间被降解而产生的阶梯状条带;末端脱氧核糖核酸转移酶介导的3^-OH末端标记法检测结果为阳性。

关键词： 羟自由基; 细胞凋亡; 烟草; DNA阶梯状条带; TUNEL

Hydroxyl radicals induce apoptosis of tobacco cells

Xia Huili, Chen Haoming, Wu Yi, Dai Yaoren

Department of Biological Sciences and Biotechnology,Tsinghua University.Beijing 100084

Abstract

Under the same conditions, other hallmarks of apoptosis including condensation and peripheral distribution of nuclei chromatin, collapse of nuclei and positive reaction to the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) procedure were also detected.

Key words: Hydroxyl radical;Apoptosis;Nicotiana tabacum;DNA ladder;TUNEL

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引用本文：

夏慧莉, 陈浩明, 吴逸, 戴尧仁. 羟自由基诱导烟草细胞凋亡[J]. 生理学报 1999; 51 (4): .

Xia Huili, Chen Haoming, Wu Yi, Dai Yaoren. Hydroxyl radicals induce apoptosis of tobacco cells. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).