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蓝光对绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响

王晓明, 王小菁, 潘瑞炽

华南师范大学生物系植物生理研究室.广东,广州 510631

摘要

蓝光、白光和黑暗对绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响不同。培养后3-18d,蓝光处理材料的可溶性蛋白质含量明显高于白光处理,更高于黑暗培养的材料。蓝光和白光明显促进~(3)H-亮氨酸掺入蛋白质,而蓝光和白光处理后游离氨基酸含量与黑暗对照相比,下降时间早,幅度大。在培养过程中,蛋白酶活性的变化与游离氨基酸相似。蛋白质合成抑制剂环己酰亚胺(CHM)抑制愈伤组织生长,其中以蓝光最大,白光次之,黑暗最小。在培养基中加入CHM愈早,抑制程度愈大。实验表明,CHM抑制愈伤组织蛋白质合成,也是以蓝光最甚。由此可见,蓝光促进绿豆下胚轴愈伤组织的形成、生长和蛋白质合成。

关键词： 绿豆; 蓝光; 愈伤组织; 蛋白质

Effects of blue light on protein metabolism during the formation and growth of mung bean hypocotyl calli

Wang Xiaoming, Wang Xiaojing, Pan Ruichi

Department of Biology,South China Normal University.Guangzhou 510631,Guangdong

Abstract

The effect of blue light on protein metabolism during the formation and growth of mung bean hypocotyl calli was investigated. The results obtained by measuring the protein contents, the incorporation of ~(3)H-leucine into protein and the proteinase activity demonstrated that 3 to 18 days after incubation, protein content in the explants grown under blue light was the highest. On the contrary, free amino acids in the tissue of blue and white light treatments declined faster and their contents were lower than those grown in the dark. The activity of proteinase was inhibited both by blue and white lights. Moreover, incorporation of ~(3)H-leucine into protein was strongly enhanced by blue and white light.

Key words: Mung bean;Blue light;Callus;Protein

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引用本文：

王晓明, 王小菁, 潘瑞炽. 蓝光对绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响[J]. 生理学报 1999; 51 (4): .

Wang Xiaoming, Wang Xiaojing, Pan Ruichi. Effects of blue light on protein metabolism during the formation and growth of mung bean hypocotyl calli. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).