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提高小麦愈伤组织分化频率的因素

于晓红, 朱祯, 付志明, 安利佳, 李向辉

中科院遗传研究所.北京 100101；辽宁师范大学.辽宁,大连 116029

摘要

在愈伤组织诱导和继代过程中添加ABA(1.0mg/L)有利于小麦中晚期幼胚致密愈伤组织的诱导及再生能力的保持;外植体来源尤其的基因型对长期培养的愈伤组织再生能力有很大影响;不同的外源激素(KT、6-BA、IAA、TDZ和玉米素等)也影响芽分化频率,其中TDZ可明显提高芽分化频率;在转入分化培养前对愈伤组织进行干燥处理可有效地提高其芽分化频率;在生根培养基中添加适量的IAA或NAA可有效促进生根。

关键词： 小麦; 愈伤组织诱导; 芽分化; 植株再生

Improvement in plant regeneration from calus in wheat

Yu Xiaohong, Zhu Zhen, Fu Zhiming, An Lijia, Li Xianghui

Institute of Genetics,Chinese Academy of Sciences.Beijing 100101；China

Abstract

The results indicated: supplementing the induction and subculture medium with ABA (1.0mg/L) increased the development of embryogenic callus from midlate immature embryos; Plant source especially genotype played an important role on the bud differentiatio nof long-term cultured callus (bid formation rate differs much between calli coming from plants of different genotypes); Different hormones (KT, IAA, NAA, TDZ, 6-BA and ZT, etc.) affected the bud differentiation frequency, among them, TDZ increased the frequency of shoot formation. Proper partial desiccation of callus before transferred to regeneration medium was found to improve the frequency of plant differentiation. Supplementing with suitable concentration of IAA or NAA in rooting medium could strengthen root formation.

Key words: Wheat;Callus induction;Bud differentiation;Plant regeneration

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引用本文：

于晓红, 朱祯, 付志明, 安利佳, 李向辉. 提高小麦愈伤组织分化频率的因素[J]. 生理学报 1999; 51 (4): .

Yu Xiaohong, Zhu Zhen, Fu Zhiming, An Lijia, Li Xianghui. Improvement in plant regeneration from calus in wheat. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).