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不同基因型玉米的再生能力和胚性与非胚性愈伤组织DNA的差异

黄璐, 卫志明

中科院上海植物生理研究所.上海 200032

摘要

低浓度(0.2mg/L)的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生,6-BA的效果比KT更好。不同品种的玉米幼胚诱导的愈伤组织的再生能力差异显著,普甜1号和苏玉1号的再生频率高达78%和75%,糯玉米和掖单9号仅为10%和8%。植株再生途径也有所不同,普甜1号以器官发生为主要途径,苏玉1号则以体胚发生途径为主。经长期继代的愈伤组织失去再生能力,通过RAPD方法比较发现胚性与非胚性愈伤组织的基因组之间存在差异,说明组织培养过程中愈伤组织的DNA发生了变异。

关键词： 玉米; 愈伤组织; 植株再生; RAPD

Differences in regeneration capacity between five elite varieties of maize (Zea mays L.) and the DNA differences between embryogenic callus and nonembryogenic callus

Huang Lu, Wei Zhiming

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Cytokinin KT and 6-BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6-BA was more effective than KT. Five maize (Zea mays L.) varieties of commercial importance were screened for the regeneration capacity of calluses formed from their immature embryo. The difference in the rate of regeneration between 78% of Putian No. 1 and 8% of Yedan No. 9 were pronounced. The mode of plant regeneration from type I callus also varied with the genotypes. Suyu No. 1 tended to regenerate via somatic embryogenesis, while Putian No. 1 was likely to regenerate via organogenesis. Maize callus lost the capacity of embryogenesis during long-time subculture. The results of randomly amplified polymorphic DNA (RAPD) showed that its genome DNA was modified in tissue culture.

Key words: Zea mays L.;Callus;Plant regeneration;RAPD

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引用本文：

黄璐, 卫志明. 不同基因型玉米的再生能力和胚性与非胚性愈伤组织DNA的差异[J]. 生理学报 1999; 51 (4): .

Huang Lu, Wei Zhiming. Differences in regeneration capacity between five elite varieties of maize (Zea mays L.) and the DNA differences between embryogenic callus and nonembryogenic callus. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).