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稻瘟病菌(Magnaporthe grisea)诱导的水稻早期反应基因ER1的5^端cDNA片段克隆及序列分析

周建明, 朱群, 白永延

中科院上海植物生理研究所.上海 200032；Salk Institute for Biological Studies.CA 92037

摘要

关键词： 水稻; 稻瘟病菌; ER1基因; 5’cDNA片段; 5’RACE

Cloning and sequencing of 5^-end cDNA fragment of early response gene (ER1) of rice induced by the blast fungus Magnaporthe grisea

Zhou Jianming, Zhu Qun, Bai Yongyan

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032；USA

Abstract

The 5^-end cDNA fragment (ER1^) of early responsive gene (ER1) of rice (Oryza sativa L. subsp. japonica No. 4) induced by blast fungus M. grisea has been isolated and sequenced (606 bp) by 5^ RACE technique. DNA sequence analysis showed that ER1^ (606 bp) can encode 134 amino acids and there are 309 bp nucleotides located at the 3^-end of the untranslated region of mRNA of ER1 gene. The 89 bp overlapping fragment was found between the 5^-end of ER1 cDNA fragment and the 3^-end of ER1^. The partial indentities of amino acid sequence deduced from ER1^ with an unknown protein (gene accession number AC002409) of Arabidopsis thaliana was 82%.

Key words: Rice;Magnaporthe grisea;ER1 gene;5' end cDNA (ER1');5'RACE

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引用本文：

周建明, 朱群, 白永延. 稻瘟病菌(Magnaporthe grisea)诱导的水稻早期反应基因ER1的5^端cDNA片段克隆及序列分析[J]. 生理学报 1999; 51 (4): .

Zhou Jianming, Zhu Qun, Bai Yongyan. Cloning and sequencing of 5^-end cDNA fragment of early response gene (ER1) of rice induced by the blast fungus Magnaporthe grisea. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).