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一种水稻酰基辅酶A结合蛋白cDNA的鉴定

张明永, 徐是雄, Chye M-L, 梁承邺

中科院华南植物研究所.广东,广州 510650；香港大学植物系.香港

摘要

对一水稻cDNA克隆(R1908)的分析表明,其可能编码水稻酰基辅酶A结合蛋白(acyl-CoA-binding protein,ACBP)。Southern杂交显示水稻(Oryza sativa L.)基因组中仅有一个该基因的拷贝。Northern分析表明水稻的ACBP基因在水稻的根、茎、叶、叶鞘、黄化苗和幼穗中皆表达,而以黄化苗的绿苗叶鞘中的表达强度高于绿苗叶片。

关键词： 水稻; 酰基辅酶A结合蛋白; cDNA

Identification of a rice cDNA encoding the acyl-CoA-binding protein (ACBP)

Zhang Mingyong, Xu Shixiong, Chye M-L, Liang Chengye

South China Institute of Botany,Chinese Academy of Sciences.Guangzhou 510650,Guangdong

Abstract

The cDNA possibly encoding the acyl-CoA-binding protein (ACBP) of rice (Oryza sativa L.) and its expressions were studied. Southern analysis suggested only one copy of ACBP gene in rice genome; Northern analysis showed that rice ACBP gene expressed in all rice organs examined (including roots, leaves, shoots, leaf sheaths of 50 d plants and young panicles), but the level of ACBP gene expression in roots of seedlings and leaf sheaths of 50 d plants was higher than in leaves.

Key words: Rice;Acyl CoA binding protein;cDNA

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引用本文：

张明永, 徐是雄, Chye M-L, 梁承邺. 一种水稻酰基辅酶A结合蛋白cDNA的鉴定[J]. 生理学报 1999; 51 (4): .

Zhang Mingyong, Xu Shixiong, Chye M-L, Liang Chengye. Identification of a rice cDNA encoding the acyl-CoA-binding protein (ACBP). Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).