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反义MAPK寡核苷酸对AngⅡ及EGF诱导的心肌成纤维细胞增殖的抑制效应

丁波, 黄韶铃, 李云霞

湖南医科大学心血管生理研究室.湖南,长沙 410078；湖南医科大学分子药理研究室

摘要

研究丝裂原活化蛋白激酶(MAPK)在血管紧张素Ⅱ(AngⅡ)的表皮生长因子(EGF)诱导的心肌成纤维细胞(FB)分裂反应中的作用及反义MAPK寡核苷酸的分裂作用和机制。结果提示,在AngⅡ或EGF诱导的心肌FB分裂反应中均有MAPK激活过程参与,而反义MAPK ODN通过阻断MAPK蛋白表达抑制AngⅡ或EGF诱导的分裂反应。

关键词： 心肌成纤维细胞; 分裂; 血管紧张素Ⅱ; 表皮生长因子; 丝裂素活化蛋白激酶; 反义寡脱氧核苷酸

Inhibitory effects of antisense oligonucleotides targeting mitogen-activated protein kinase (MAPK) mRNA on neonatal rat cardiac fibroblast proliferation induced by AngII and EGF

Ding Bo, Huang Shaoling, Li Yunxia

Laboratory of Cardiovascular Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

In the present study, the antisense oligodeoxynucleotide (ODN) approach was used to investigate whether mitogen-activated protein kinase (MAPK) is necessary for the proliferation response in neonatal rat cardiac fibroblast (FB) induced by angiotensin II (AngII) or epidermal growth factor (EGF), the proximal cytosolic signal transduction pathways which are quite different processes. A phosphorothioate0protected 17-mer directed against the initiation of translation sites P~(42) MAPK mRNA was introduced into FB by liposomal transfection.

Key words: Cardiac fibroblast;Proliferation angiotensin Ⅱ;Epidermal growth factor;Mitogen activated protein kinase;Antisense oligonucleotide;

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引用本文：

丁波, 黄韶铃, 李云霞. 反义MAPK寡核苷酸对AngⅡ及EGF诱导的心肌成纤维细胞增殖的抑制效应[J]. 生理学报 1999; 51 (4): .

Ding Bo, Huang Shaoling, Li Yunxia. Inhibitory effects of antisense oligonucleotides targeting mitogen-activated protein kinase (MAPK) mRNA on neonatal rat cardiac fibroblast proliferation induced by AngII and EGF. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).