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P物质对大鼠骶髓后连合核神经元甘氨酸激活的氯电流的调控

王殿仕, 徐天乐, 李继硕

第四军医大学基础部解剖学教研室,梁qiu锯脑研究中心.陕西,西安 710032；中国科学技术大学生命科学学院神经生物学与生物物理学系.安徽,合肥 230027

摘要

采用制霉菌素穿孔膜片箝技术,研究了P物质(substance P,SP)对急性分离的大鼠骶髓后连合核神经元士的宁敏感性甘氨酸(glycine,Gly)反应的调控作用。蛋白激酶C或钙离子/钙调素依赖性的蛋白激酶Ⅱ可能参与了SP对I_(Gly)增强作用的胞内机制。结果提示,SP可能通过增强Gly的反应而抑制脊髓伤害性信息的传导。

关键词： P物质; 甘氨酸; 骶髓后连合核; 蛋白激酶C; 钙离子/钙调素依赖性的蛋白激酶Ⅱ

Modulation of glycine-activated chloride currents by substance P in rat sacral dorsal commissural neurons

Wang Dianshi, Xu Tianle, Li Jishuo

Department of Anatomy and K. K. Leung Brain Research Center,Fourth Military Medical University.Xi^an 710032,Shaanxi；China

Abstract

The modulatory effect of substance P (SP) on strychnine-sensitive glycine (Gly) response was examined in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using nystatin perforated patch recording configuration under voltage-clamp conditions. The facilitatory action of SP on I_(Gly) could also be abolished by pretreatment with chelerythrine or KN-62 in different neurons, a finding suggesting that protein kinase C (PKC) or Ca~(2+)/calmodulin-dependent protein kinase II (CaMKII) possibly contributes to an intracellular pathway of SP in the augmentation of I_(Gly). The results imply that SP may suppress nociception in the spinal cord by potentiating Gly response.

Key words: Substance P;Glycine;Sacral dorsal commissural nucleus;Protein kinase C;Ca~(2+)/calmodulin dependent protein kinase Ⅱ

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引用本文：

王殿仕, 徐天乐, 李继硕. P物质对大鼠骶髓后连合核神经元甘氨酸激活的氯电流的调控[J]. 生理学报 1999; 51 (4): .

Wang Dianshi, Xu Tianle, Li Jishuo. Modulation of glycine-activated chloride currents by substance P in rat sacral dorsal commissural neurons. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).