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巢蛋白在P19神经元分化过程中的表达

边玮, 杨靖, 唐珂, 景乃禾

中科院上海生物化学研究所.上海 200031

摘要

巢蛋白基因的表达早于神经前体细胞中表达的BMP4,以及在成熟神经元中特异表达的标记分子神经丝(NF160),表明巢蛋白基因可能主要在神经干细胞阶段表达。细胞免疫染色结果显示,巢蛋白在神经突及生长锥上存在较强的分布,表明它可能参与了有丝分裂后神经元与靶细胞之间神经联系的建立。

关键词： 巢蛋白; P19神经元; BMP4; 神经丝

Nestin expression during P19 neuron differentiation

Bian Wei, Yang Jing, Tang Ke, Jing Naihe

Shanghai Institute of Biochemistry,Chinese Academy of Science.Shanghai 200031

Abstract

Mouse nestin, an intermediate filament gene, is transiently expressed during the development of the central nervous system. In order to find the clue of its function during neural development, we tried to find out the gene expression pattern during the neuronal differentiation of P19 EC cells induced RA. RT-PCR showed that nestin was transiently expressed during P19 neuron differentiation, with a peak at day 4 of this process. However, BMP4, a neural precursor cell marker, was transiently expressed with its highest level at day 6, while NF160 kD a terminal differentiated neuronal marker, was increasingly expressed during the whole process.

Key words: Nestin;P19 neuron;BMP4;Neural filament

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引用本文：

边玮, 杨靖, 唐珂, 景乃禾. 巢蛋白在P19神经元分化过程中的表达[J]. 生理学报 1999; 51 (3): .

Bian Wei, Yang Jing, Tang Ke, Jing Naihe. Nestin expression during P19 neuron differentiation. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).