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血管平滑肌细胞增殖与Cdk抑制蛋白p27的表达

袁勇, 许顶立, 刘伊丽, 贾满盈

第一军医大学南方医院心内科.广东,广州 510515

摘要

AngⅡ刺激血管平滑肌细胞(VSMCs)增生,其蛋白含量增加了43.6%(P<0.01),但不抑制p27蛋白的表达;AVP可轻度抑制p27的表达,有轻度促进VSMCs增殖和增生的作用(P<0.05);血小板源生长因子(PDGF)明显抑制p27的表达,引起细胞增殖。结果提示,p27蛋白抑制VSMCs通过G_(1)期进入S期,是抑制VSMCs增殖的重要调节因子。

关键词： p27蛋白; 细胞周期; 血管平滑肌细胞; 流式细胞仪

Expression of cyclin dependent kinase inhibitor p27 during proliferation in vascular smooth muscle cell

Yuan Yong, Xu Dingli, Liu Yili, Jia Manying

Department of Cardiology,Nanfang Hospital,The First Military Medical University.Guangzhou 510515,Guangdong

Abstract

Deprived of fotal calf serum for 48 h, cultured VSMCs in quiescent condition were collected at different times after stimulation of AngⅡ, AVP and PDGF-BB. Cell cycle distribution and p27 expression were determined with a flow cytometer. The results showed that the protein content of VSMCs was significantly increased (43.6%) by AngⅡ as a result of hypertrophy, but AngⅡ did not lead to downregulation of p27. AVP could downregulate p27 slightly. PDGF could inhibit p27 expression significantly and cause VSMCs hyperplasia.

Key words: p27;Cell cycle;Vascular smooth muscle cell;Flow cytometer

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引用本文：

袁勇, 许顶立, 刘伊丽, 贾满盈. 血管平滑肌细胞增殖与Cdk抑制蛋白p27的表达[J]. 生理学报 1999; 51 (3): .

Yuan Yong, Xu Dingli, Liu Yili, Jia Manying. Expression of cyclin dependent kinase inhibitor p27 during proliferation in vascular smooth muscle cell. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).