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人SOD_(1)基因在大鼠血管平滑肌细胞的表达及对抗氧自由基损伤的作用

郑新程, 安威, 白景香, 毛松华, 伍贻经

北京医科大学病理生理教研室.北京 100083；首都医科大学细胞生物系.北京 100054；北京医科大学人民医院普外科.北京 100035

摘要

构建含人铜锌超氧化物歧化酶(hSOD_(1))基因的逆转录病毒载体,将其导入离体培养的鼠血管平滑肌细胞(VSMCs),观察hSOD_(1)基因表达及其抗氧自由基损害作用。结果提示,外源性表达的SOD可能通过抗自由基损伤和抑制内皮细胞增殖两方面作用阻止动脉粥样硬化的形成。

关键词： 超氧化物歧化酶; 基因; 血管平滑肌细胞; 自由基

Expression of human superoxide dismutase gene in rat vascular smooth muscle cells and its anti-oxidative effect

Zheng Xincheng, An Wei, Bai Jingxiang, Mao Songhua, Wu Yijing

Department of Pathophysiology,Beijing Medical University.Beijing 100083；China；Department of General Surgery,The People^s Hospital of Beijing Medical University.Beijing 100035

Abstract

A retroviral vector containing human superoxide dismutase (SOD) cDNA was constructed and transfected into rat vascular smooth muscle cells (VSMCs). The expression of exogenous hSOD_(1) in the VSMCs was analyzed with Northern and Southern blot. The protection of the transfected and/or non-transfected VSMCs from free radical produced by the xanthine/xanthine oxidase (X/XO) system was investigated. It is suggested that the expression of gene-transferred hSOD_(1) is able to prevent the formation of atherosclerosis, partially due to its cell protection and inhibition of the proliferative embolization.

Key words: Superoxide dismutase (SOD);Gene;Vascular smooth muscle cells;Free radical

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引用本文：

郑新程, 安威, 白景香, 毛松华, 伍贻经. 人SOD_(1)基因在大鼠血管平滑肌细胞的表达及对抗氧自由基损伤的作用[J]. 生理学报 1999; 51 (2): .

Zheng Xincheng, An Wei, Bai Jingxiang, Mao Songhua, Wu Yijing. Expression of human superoxide dismutase gene in rat vascular smooth muscle cells and its anti-oxidative effect. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).