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萌发中花生胚轴的耐干性与热稳定蛋白

黄上志, 王冬梅, 卢春斌, 傅家瑞

中山大学生物科学与技术系.广东,广州 510275

摘要

成熟花生种子吸胀18h发芽率达100%。在这18h的范围内,胚轴即使经干燥处理,萌发生长率仍保持100%,而热稳定蛋白含量变化很小。吸胀24h后,经干燥的花生胚完全丧失萌发生长能力。SDS-PAGE和双向电泳表明,花生胚轴的热稳定蛋白主要是贮藏蛋白,该蛋白中的花生球蛋白大亚基,伴花生球蛋白I和2S蛋白的降解与胚轴的耐干性丧失有关。

关键词： 花生种子; 耐干性; 热稳定蛋白; 贮藏蛋白

Heat-stable proteins and desiccation tolerance in axes of germinating peanut seeds

Huang Shangzhi, Wang Dongmei, Lu Chunbin, Fu Jiarui

Department of Bioscience & Biotechnology,Zhongshan University.Guangzhou 510275,Guangdong

Abstract

Peanut (Arachis hypogaea L.) seed reached 100% germination at 18h after imbibition. Peanut axes from seeds undergoing for less than 18h and subjected to desiccation maintained 100% germinability, but those from seeds imbibing for 24h had zero germinability. During germination, the content of heat-stable proteins in axes lowered slowly. After 24h imbibition, the content of heat-stable proteins in axes declined remarkably. SDS-PAGE and two dimensional electrophoresis showed that the heat-stable proteins in peanut axes consisted mainly of storage proteins. The degradation of the major subunits of arachin, conarachin I and 2S proteins were related to the loss of desiccation tolerance in germinated peanut axes.

Key words: Peanut seed;Desiccation tolerance;Heat-stable protein;Storage protein

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引用本文：

黄上志, 王冬梅, 卢春斌, 傅家瑞. 萌发中花生胚轴的耐干性与热稳定蛋白[J]. 生理学报 1999; 51 (2): .

Huang Shangzhi, Wang Dongmei, Lu Chunbin, Fu Jiarui. Heat-stable proteins and desiccation tolerance in axes of germinating peanut seeds. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).