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光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响

赵德修, 李茂寅, 邢建民, 童哲

中科院植物研究所.北京 100093

摘要

白光、蓝光、红光和远红光等不同光质照射及不同的白光强度对水母雪莲愈伤组织生长、苯丙氨酸氨基裂解酶(PAL)活性及黄酮合成有不同的影响。红光明显促进愈伤组织的生长,但强烈抑制PAL活性和黄酮的合成。蓝光对愈伤组织生长无明显影响,但显著提高PAL活性和黄酮的合成。白光的作用介于红光和蓝光之间。远红光的作用与蓝光相似,但作用比较弱。每天16h蓝光加8h白光和8h蓝光加16h白光的组合产生最高的黄酮含量和黄酮生产率。

关键词： 水母雪莲; 愈伤组织培养; 苯丙氨酸氨基裂解酶; 黄酮; 光质; 光辐照度

Effects of light on cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim

Zhao Dexiu, Li Maoyin, Xing Jianmin, Tong Zhe

Institute of Botany,Chinese Academy of Sciences.Beijing 100093

Abstract

Effects of white light(WL)、blut light(BL)、red light(RL) a nd farred light (FRL) on callus growth, flavonoids biosynthesis and phenylalanine ammonialyase (PAL) activity in the culture of Saussurea medusa Maxim. on solid medium were examined. The results showed that the growth of callus cells was significantly promoted by continuous RL, and moderately by WL and FRL, BL had nearly no effect on the cell growth, compared with that in darkness. However, BL had stronger promotion on PAL activity and flavonoids biosynthesis. The effects of WL on callus growth, PAL activity and flavinoids accumulation were between those of RL and BL. PAL activity and flavonoids accumulation increased as WL fluence rate was raised from 0 to 60#mu#mol m~(-2)s~(-1) while cell growth ch anged little. Experiments with different duration of illumination in a day revealed that PAL activities and flavonoids contents were promoted with the increase of irradiation duration of BL or WL. The experiments with diurnal alteration of WL and BL irradiation indicated that 16h WL/8h BL and 8h WL/16h BL diurnal cycles produced the largest total amount of flavonoids.

Key words: Saussurea medusa Maxim.;callus culture;Phenylalanine ammonialyase;Flavonoids;Light quality;Fluence rate

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引用本文：

赵德修, 李茂寅, 邢建民, 童哲. 光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响[J]. 生理学报 1999; 51 (2): .

Zhao Dexiu, Li Maoyin, Xing Jianmin, Tong Zhe. Effects of light on cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).