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用量热法研究水稻线粒体不同条件下的能量释放

周培疆, 凌杏元, 周涵韬, 屈松生, 朱英国

武汉大学生命科学学院.湖北,武汉 430072；武汉大学化学学院.湖北,武汉 430072

摘要

用精密微热量计(LKB-2277生物活性检测系统)和差示扫描量热(differential scanning calorimetry,DSC)仪(Dupont-910)分别测定了广丛41B水稻线粒体体外能量释放热谱和DSC曲线。探讨了它在恒温和变温条件下能量释放的规律和特性,建立了广丛41B水稻线粒体在不同条件下能量释放的动力学模型,计算了它在能量释放过程中的热力学和动力学参数,并比较了它们之间的差异。结果表明,温度愈低,线粒体能量释放速率愈慢。线粒体在零上低温下放置较长时间后,其放热量增加,能量释放速率加快。

关键词： 水稻; 线粒体; 能量释放; 量热; 热力学; 动力学

Studies on the energy liberation of rice mitochondria under different conditions by means of calorimetry

Zhou Peijiang, Ling Xingyuan, Zhou Hantao, Qu Songsheng, Zhu Yingguo

College of Life Sciences,Wuhan University.Wuhan 430072,Hubei；China

Abstract

This paper reported the thermodynamic and kinetic behaviours of the energy liberation of the mitochondria isolated from Guangchong 41 fertile lines of rice (Oryza sativa L.) studied by using an LKB-2277 bioactivity monitor and a Dupont-910 differential scanning calorimeter (DSC) & 9900 computer/thermal analyzer system under constant and changing temperature. The thermograms of energy liberation of the rice mitochondria (which had been kept at 0-3℃ for 15h and 40d) were determined respectively at 25 and 30℃, and the difference in shape of the thermograms and thermodynamic and kinetic characteristics were compared. The results indicated that the lower the temperature, the slower the energy liberation rate of the rice mitochondria. Both the heat releast and the energy liberation rate of the rice mitochondria increased after the mitochondria had been kept at lower temperature for long time. In this work, authors also determined the DSC curve of the rice mitochondrial energy liberation under changing temperature, and calculated the thermodynamic and thermodynamic and kinetic characteristic parameters of the rice mitochondrial energy liberation under changing temperature.

Key words: Rice(Oryza sativa L.);Mitochondria energy liberation;Calorimetry;Thermodynamics;Kinetics;

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引用本文：

周培疆, 凌杏元, 周涵韬, 屈松生, 朱英国. 用量热法研究水稻线粒体不同条件下的能量释放[J]. 生理学报 1999; 51 (2): .

Zhou Peijiang, Ling Xingyuan, Zhou Hantao, Qu Songsheng, Zhu Yingguo. Studies on the energy liberation of rice mitochondria under different conditions by means of calorimetry. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).