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抑制素#alpha#亚基片段P~(33)对大鼠离体培养黄体细胞凋亡的影响

张江红, 俞瑾, 丰有吉, 倪江, 程治平, 孙颖, 鲁桂琛, 吴燕婉, 李伟雄

上海医科大学妇产科研究所.上海 200011；哈尔滨医科大学生理教研室.黑龙江,哈尔滨 150086；医科院药物研究所.北京 100050；北京国家计划生育科学技术研究所.北京 100083

摘要

进一步在细胞水平探讨P~(33)促进黄体细胞凋亡的作用机制。应用DNA电泳检测技术、DNA荧光(AO-EB PI)染色和流式细胞分析方法观察了P~(33)对PMSG-hCG假孕大鼠胶原酶DNA酶分散的黄体细胞的自发凋亡的影响。结果表明,P~(33)促进培养大鼠黄体细胞的自发凋亡,其作用机制可能与TPK途径有关。

关键词： 凋亡; 黄体细胞; 抑制素α亚基片段; 酪氨酸蛋白激酶; RNA合成; 蛋白质合成; 大鼠

Effect of human inhibin #alpha#-fragment 1-32-tyr (P~(33)) on apoptosis of cultured rat corpus luteal cells

Zhang Jianghong, Yu Jin, Feng Youji, Ni Jiang, Cheng Zhiping, Sun Ying, Lu Guichen, Wu Yanwan, Li Weixiong

Institute of Obstetrics/Gynecology,Shanghai Medical University.Shanghai 200011；China；Institute of Materia Medica,Chinese Academy of Sciences.Beijing 100050

Abstract

In the present work, the action of P~(33) on apoptosis was further studied in vitro in cultured rat CL cells. Gel electrophoretic analysis for detection of oligonucleosomal DNA fragmentation, AO-EB or PI assays and flow cytometry were used to observe the action of P~(33) on the occurrence of spontaneous apoptosis by collegenase-DNase dispersed CL cells, obtained from PMSG-hCG induced pseudopregnent rats. The results suggest that P~(33) stimulates spontaneous apoptosis in cultured rat CL cells with the involvement of tyrosine specific protein kinase system.

Key words: Apoptosis;Corpus luteal cells;Inhibin #alpha#-subunit fragment;Tyrosine specific protein kinase (TPK);RNA synthesis;Protein synthesis rat;

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引用本文：

张江红, 俞瑾, 丰有吉, 倪江, 程治平, 孙颖, 鲁桂琛, 吴燕婉, 李伟雄. 抑制素#alpha#亚基片段P~(33)对大鼠离体培养黄体细胞凋亡的影响[J]. 生理学报 1999; 51 (1): 87-95.

Zhang Jianghong, Yu Jin, Feng Youji, Ni Jiang, Cheng Zhiping, Sun Ying, Lu Guichen, Wu Yanwan, Li Weixiong. Effect of human inhibin #alpha#-fragment 1-32-tyr (P~(33)) on apoptosis of cultured rat corpus luteal cells. Acta Physiol Sin 1999; 51 (1): 87-95 (in Chinese with English abstract).