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促肾上腺皮质激素对慢痛大鼠脊髓生长抑素、c-fos表达及痛反应的影响

武强, 李希成, 阮怀珍, 黎海蒂

第三军医大学生理教研室.重庆 400038

摘要

足底注射甲醛可使大鼠脊髓内c-fos样免疫反应(FLI)、Som样免疫反应(Som-LI)、FLI/Som-LI及前Som原mRNA(PPS-mRNA)神经元数目显著增多以及痛级均数(PIR)显著升高。而鞘内注射ACTH可显著抑制甲醛引起的大鼠脊髓内FLI、Som-LI、FLI/Som-LIL及PPS-mRNA增多和PIR升高效应。鞘内预先注射赛庚啶可阻断ACTH的抑制效应,而荷包牡丹碱、纳洛酮则无影响。

关键词： ACTH; 生长抑素; 原癌基因c-fos; 脊髓; 免疫组化; 原位杂交

Effect of ACTH on the expression of somatostatin and c-fos in the spinal cord and formalin evoked pain response

Wu Qiang, Li Xicheng, Ruan Huaizhen, Li Haidi

Department of Physiology,Third Military Medical University.Chongqing 400038

Abstract

Subcutaneous injection of formalin in the right hindpaw increased c-fos-like immunoreactivity (FLI), somatostatin-like immunoreactivity (Som-LI), Som-LI/FLI and perprosomatostatin mRNA (PPS-mRNA) in neurones of right spinal dorsal horn and significantly enhanced pain intensity rating. ACTH decreased the FLI, Som-LI, Som-LI/FLI and PPS-mRNA levels of the spinal cord evoked by formalin. The decrease of c-fos or Som level due to intrathecal injection of ACTH in rats with chronic pain was prevented by injection of cyproheptadine, but not by bicuculline and naloxone.

Key words: ACTH;Somatostatin;Proto oncogene c-fos;Spinal cord;Immunohistochemistry;In situ hybridization

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引用本文：

武强, 李希成, 阮怀珍, 黎海蒂. 促肾上腺皮质激素对慢痛大鼠脊髓生长抑素、c-fos表达及痛反应的影响[J]. 生理学报 1999; 51 (1): 60-64.

Wu Qiang, Li Xicheng, Ruan Huaizhen, Li Haidi. Effect of ACTH on the expression of somatostatin and c-fos in the spinal cord and formalin evoked pain response. Acta Physiol Sin 1999; 51 (1): 60-64 (in Chinese with English abstract).