过刊浏览

糖皮质激素对大鼠肾上腺髓质嗜铬细胞瘤细胞ACh诱发电流的快速抑制作用

石丽君, 王春安, 陈宜张

第二军医大学基础医学部生理学教研室.上海 200433

摘要

该研究采用全细胞膜片箝技术,以大鼠肾上腺髓质嗜铬细胞瘤细胞(PC12)为标本,观察了糖皮质激素(GC)对乙酰胆碱诱发电流(I_(ACh))的快速作用,并初步探讨了其可能机制。结果提示:GC对PC12细胞上I_(ACh)有快速抑制作用,其机制可能是由非基因组机制介导的;不同GC对I_(ACh)的抑制作用不同。

关键词： 糖皮质激素; 烟碱受体; 大鼠肾上腺髓质嗜铬细胞瘤细胞; 非基因组机制

RAPID inhibitory effect of glucocorticoids on ACh-induced current in rat phaeochromocytoma cells

Shi Lijun, Wang Chunan, Chen Yizhang

Department of Physiology,Department of Basic Medicine,Second Military Medical University.Shanghai 200043

Abstract

A rapid effect of glucocorticoids (GC) on acetycholine-induced current in rat phaeochromocytoma (PC12) cells and its possible mechanism were investigated by whole-cell clamp technique. The results suggested that GC induced rapid inhibitory effects on I_(ACh) in PC12 cells are probably mediated by a nongenomic mechanism. The inhibitory effect of various GC on I_(ACh) are different.

Key words: Glucocorticoids;Nicotinic ACh receptor;Phaeochromocytoma cells;Nongenomic

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

石丽君, 王春安, 陈宜张. 糖皮质激素对大鼠肾上腺髓质嗜铬细胞瘤细胞ACh诱发电流的快速抑制作用[J]. 生理学报 1998; 50 (6): .

Shi Lijun, Wang Chunan, Chen Yizhang. RAPID inhibitory effect of glucocorticoids on ACh-induced current in rat phaeochromocytoma cells. Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).