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低氧对肺内小动脉平滑肌细胞血管紧张素转化酶活性和基因表达的影响

于天正, 马传桃

湖南医科大学心血管生理研究室.湖南,长沙 410078

摘要

实验采用分离培养兔肺内小动态平滑细胞(PASMC),观察低氧对PASMC的血管紧张素转化酶(ACE)活性和基因表达的影响。结果提示:基础状态下培养的兔PASMC存在ACE;低氧可促进PASMC ACE活性和基因表达,这可能与低氧性肺动脉高压有关。

关键词： 低氧; 肺动脉; 平滑肌细胞; 血管紧张素转化酶(ACE); 兔

Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells

Yu Tianzheng, Ma Chuantao

Laboratory of Cardiovascular Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

In order to observe the effect of hypoxia on pulmonary artery smooth muscle cells (PASMC), angiotensin-converting enzyme (ACE) activity was measured by colometry and the expression of ACE mRNA was detected by Northern blot, using rabbit isolated and cultured PASMC. The results suggest that PASMC ACE increased by hypoxia might play an important role in the development of hypoxic pulmonary hypertension.

Key words: Hypoxia;Pulmonary artery;Vascular smooth muscle cell;Angiotension converting enzyme (ACE);Rabbit

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引用本文：

于天正, 马传桃. 低氧对肺内小动脉平滑肌细胞血管紧张素转化酶活性和基因表达的影响[J]. 生理学报 1998; 50 (6): .

Yu Tianzheng, Ma Chuantao. Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells. Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).