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锌离子对急性分离的大鼠骶髓后连合核神经元GABA_(A)受体介导电流的抑制作用

庞志平, 王殿仕, 徐天乐, 李继硕

第四军医大学解剖学教研室.陕西,西安 710032

摘要

采用制霉菌素穿孔膜片箝技术,研究了锌离子(Zn~(2+))对急性分离的大鼠骶髓后连合核神经元GABA_(A)受体介导电流的作用。结果表明:在箝制电压为-40mV时,GABA可通过GABA_(A)受体介导产生内向电流;此电流可被Zn~(2+)呈非电压依赖性可逆地阻断;在Zn~(2+)存在的情况下,GABA的浓度-效应曲线平行右移。

关键词： Zn~(2+); GABA_(A)受体; 制霉菌素穿孔全细胞记录; 分离细胞; 骶髓后连合核

Zn~(2+) depresses GABA_(A) receptor mediated responses in acutely dissociated sacral dorsal commissural neurons

Pang Zhiping, Wang Dianshi, Xu Tianle, Li Jishuo

Department of Anatomy and K.K.Leung Brain Research Centre,Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

The effects of Zn~(2+) on GABA_(A)-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques. The results demonstrated that GABA induced inward currents through activation of GABA_(A)-receptor at a holding potential of -40mV; GABA_(A)-receptor mediated responses were suppressed by Zn~(2+) in a reversible and voltage-independent manner; and in the presence of Zn~(2+), the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner.

Key words: Zinc;GABA_(A)-receptor;Nystatin-perforated whole cell recording;Dissociated cells;Sacral dorsal commissura nucleus

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引用本文：

庞志平, 王殿仕, 徐天乐, 李继硕. 锌离子对急性分离的大鼠骶髓后连合核神经元GABA_(A)受体介导电流的抑制作用[J]. 生理学报 1998; 50 (6): .

Pang Zhiping, Wang Dianshi, Xu Tianle, Li Jishuo. Zn~(2+) depresses GABA_(A) receptor mediated responses in acutely dissociated sacral dorsal commissural neurons. Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).