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谷氨酸对嗜铬细胞瘤细胞热休克蛋白79mRNA的诱导分析

吴炳义, 娄淑杰, 徐仁宝, 陈宜张

第二军医大学生理学教研室.上海 200433；第二军医大学病理生理学教研室.上海 200433

摘要

在一定的浓度(50-500#mu#mol/L)和作用时间内(5-30min)谷氨酸能够引起hsp70 mRNA的诱导,而且是部分地通过其NMDA受体。乙酰胆碱(0.1-1 000#mu#mol/L)不能诱导hsp70 mRNA的增加。

关键词： 热休克蛋白; 神经递质; 鼠嗜铬细胞瘤细胞; 谷氨酸; 乙酰胆碱

Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells

Wu Bingyi, Lou Shujie, Xu Renbao, Chen Yizhang

Department of Physiology,Second Military Medical University.Shanghai 200433；China

Abstract

In the present study, the effects of glutamic acid and acetylcholine (ACh) on the induction of hsp70 mRNA in PC12 cells were studied by Northern blot method. The probe used is specific for inducible hsp70 mRNA. Our results showed that the glutamic acid under limited conditions (such as at 50-500#mu#mol/L and action time 5-30min) could induce the expression of hsp70 mRNA, which was partly mediated by NMDA receptors. On the other hand, ACh (0.1-1 000#mu#mol/L) could not induce the expression of hsp 70 mRNA.

Key words: Heat shock proteins;Neurotransmitters;PC12 cells;Glutamic acid;Acetylcholine

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引用本文：

吴炳义, 娄淑杰, 徐仁宝, 陈宜张. 谷氨酸对嗜铬细胞瘤细胞热休克蛋白79mRNA的诱导分析[J]. 生理学报 1998; 50 (5): .

Wu Bingyi, Lou Shujie, Xu Renbao, Chen Yizhang. Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells. Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).