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一氧化氮的释放对海马脑片CA1区痫样放电的影响

摘要

在青霉素致痫脑片模型上,诱发的痫波随青霉素浓度的增加而增多,与此同时,NO释放量亦增加并存在剂量反应关系;NO合酶抑制剂7-NI及L-NNA抑制海马脑片CA1区NO的产生的同时,可部分翻转青霉素的致痫作用;NO敏感电极检测线性范围为4.5×10~(-4)-1.0×10~(-8)mol/L,可用于生物组织如脑片的NO释放量测定。

关键词： 海马脑片诱发痫波; 一氧化氮; NO敏感电极; 7-NI; L-NNA

Effect of nitric oxide release on epileptiform discharge in CA1 area of hippocampal slices

Abstract

The linear relationship range and detection limit of SNM were 4.5×10~(-4)-1.0×10~(-8)mol/L and 5.0×10~(-8)mol/L respectively. Penicillin (PEN) could elevate NO release, the number and amplitude of stimulus-evoked field potentials spike (SEPS) in concentration-dependent manner; Both 7-NI and L-NNA depressed NO release and partly reversed and effect of penicillin.

Key words: Penicillin-induced seizure in hippocamal slice;Nitric chide NO-sensitive microelectrode;7-nitro-indazole;N~(#omega#)-nitro-L-arginine;

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引用本文：

. 一氧化氮的释放对海马脑片CA1区痫样放电的影响[J]. 生理学报 1998; 50 (5): .

. Effect of nitric oxide release on epileptiform discharge in CA1 area of hippocampal slices. Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).