过刊浏览

一种分离大鼠窦房结自律细胞的简单方法

摘要

关键词： 窦房结; P细胞; 心肌细胞; 胶原酶; 大鼠

A simple method of isolation and identification of P cells in rat sinoatrial node

Abstract

A method of isolating P cells of rat sinoatrial node by collagenase is described. The method is feasible for morphological and electrophysiological investigation, with the advantages of taking a relatively easy procedure and consuming a small amount of collagenase. The main features of action potentials of these isolated cells including clear diastolic automatic depolarization, mean maximal diastolic potential of -55mV, mean peak potential value of 58mV, and mean APD_(50) of 18ms, were similar to those of P cells in situ.

Key words: Sinoatrial node;Pacemaking cells;Myocardial cell;Collagenase;Rat

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

. 一种分离大鼠窦房结自律细胞的简单方法[J]. 生理学报 1998; 50 (4): .

. A simple method of isolation and identification of P cells in rat sinoatrial node. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).