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露花磷酸烯醇式丙酮酸羧化酶同功酶及其四级结构

唐犁

中科院上海植物生理研究所.上海 200032

摘要

关键词： 兼性景天科酸代谢植物; 露花; 磷酸烯醇式丙酮酸羧化酶; 酶的四级结构

The study of phosphoenolpyruvate carboxylase isoforms in Mesembryanthemum cordifolium

Tang Li

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Besides the major 250 kD PEPCase isoform, a 300 kD minor PEPCase form was also found in Mesembryoanthemum cordifolium leaves. By using SDS PAGE it was found that both isoforms were homodimers. The 250 kD form consisted of two 100 kD subunits, and the 300 kD form is a dimer of two 110 kD subunits. The discrepency between molecular weights of the native enzymes and their subunits was a result of the different molecular weight determinating methods. Therefore, there is no heterodimer to PEPCase in this plant. A 1 080 kD PEPCase form was found in both salt-stressed and well-watered leaf samples. This PEPCase is an octamer, and there was no diurnal fluctuation in its abundance.

Key words: Facultative CAM plant;Mesembryanthemum cordifolium;phosphoenolpyruvate carboxylase;Quarternary structrue

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引用本文：

唐犁. 露花磷酸烯醇式丙酮酸羧化酶同功酶及其四级结构[J]. 生理学报 1998; 50 (2): .

Tang Li. The study of phosphoenolpyruvate carboxylase isoforms in Mesembryanthemum cordifolium. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).