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大豆叶片质膜蛋白激酶的自身磷酸化反应

王宁宁, 吴海明, 王勇, 张自立, 朱亮基, 张韧

南开大学生物化学与分子生物学系.天津 300071；Wolongong大学.NSW2522

摘要

与Mg-ATP相比,Mn-ATP是更有效的57kD豆叶片质膜蛋白激酶自身磷酸化反应底物;钙离子可以促进该激酶的自身磷酸化反应活性,而且EGTA可以显著降低它在SDS电泳中的迁移率;预磷酸化反应实验证明57kD蛋白激酶具有多个自身磷酸化反应位点。

关键词： 大豆叶片; 质膜蛋白激酶; 自身磷酸化反应

Study on autophosphorylation of soybean leaf plasma membrane protein kinases

Wang Ningning, Wu Haiming, Wang Yong, Zhang Zili, Zhu Liangji, Zhang Ren

Biochemistry of Molecular Biology Department of Nankai University.Tianjin 300071；Australia

Abstract

The authors the in vitro autophosphorylation characteristics of soybean primary leaf plasma membrane protein kinases and the possible effect of autophosphorylation on enzyme activities. It was found that Mn-ATP was a more effective substrate than Mg-ATP for the autophosphorylation of the 57 kD protein kinase.

Key words: Soybean leaf;Plasma membrane protein kinase;Autophosphorylation

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引用本文：

王宁宁, 吴海明, 王勇, 张自立, 朱亮基, 张韧. 大豆叶片质膜蛋白激酶的自身磷酸化反应[J]. 生理学报 1998; 50 (2): .

Wang Ningning, Wu Haiming, Wang Yong, Zhang Zili, Zhu Liangji, Zhang Ren. Study on autophosphorylation of soybean leaf plasma membrane protein kinases. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).