叶绿体ATP合成酶#epsilon#亚基对菠菜叶绿体毫秒延迟发光的影响
史瑾, 魏家绵, 李德耀, 王颖君, 沈允钢
中科院上海植物生理研究所.上海 200032
摘要
在1℃低温条件下,经叶绿体ATP合成酶#epsilon#亚基处理的菠菜叶绿体毫秒延迟发光的快相显著高于对照,增加的快相不仅是由膜两侧的电位差所引起的,并且为光系统II水氧人释放的质子所促进。当温度升至25℃时,#epsilon#亚基对叶绿体毫秒延迟发光快相的影响几乎消失。牛血清白蛋白处理对此快相也有轻微的增强效应。
关键词: 叶绿体; ε亚基; 毫秒延迟发光; 快相; 质子区域化
Effect of #epsilon# subunit of chloroplast ATP synthase on the fast phase of millisecond delayed fluorescence in spinach chloroplast
Shi Jin, Wei Jiamian, Li Deyao, Wang Yingjun, Shen Yungang
Shanghai Institute of Plant Physiology,China Academy of Sciences.Shanghai 200032
Abstract
It is possible that the #epsilon# subunit changed the dynamic structure of thylakoid membrane near PSII at low temperature. To test this possibility, the effects of #epsilon# subunit on the fast phase of millisecond delayed fluorescence at different temperatures were compared. At 1 degree C, the increment of the fast phase of ms-DLE was apparent, while at 25 degree C, the increase of the fast phase of ms-DLE cause by the #epsilon# subunit is hard to be measured.
Key words: Chloroplast;#epsilon# subunit;Millisecond delayed fluorescence;Fast phase; Localized proton
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引用本文:
史瑾, 魏家绵, 李德耀, 王颖君, 沈允钢. 叶绿体ATP合成酶#epsilon#亚基对菠菜叶绿体毫秒延迟发光的影响[J]. 生理学报 1998; 50 (2): .
Shi Jin, Wei Jiamian, Li Deyao, Wang Yingjun, Shen Yungang. Effect of #epsilon# subunit of chloroplast ATP synthase on the fast phase of millisecond delayed fluorescence in spinach chloroplast. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).