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小麦K型及V型细胞质雄性不育系线粒体DNA的比较分析

李传友, 王斌

中科院遗传研究所.北京 100101

摘要

具有相同核遗传背景的小麦K型不育系K149A、V型不育系V149A及相应保持系149B的线粒体DNA,其结构显著不同, atpA、atp9、coxII、cob等线粒体功能基因有组织结构上的差异。基于“核质互作”的思路,“把雄性不育-育性恢复”作为一个整体来考虑,并重视比较不育系和杂种F_(1)代之间线粒体DNA的结构和表达的差异,所得结果对寻找和鉴定cms因子,进而阐明cms的形成机理有积极意义。

关键词： 小麦; 细胞质雄性不育; 线粒体DNA; RFLP; RAPD

Mitochondrial DNAs of cytoplsamic male sterile lines of K- and V-type in wheat

Li Chuanyou, Wang Bin

Institute of Genetics,Chinese Academy of Sciences.Beijing 100101

Abstract

The mitochondrial DNAs (mtDNAs) of cytoplasmic male sterile (cms) lines of K- and V-type with the same nuclear background were compared with that of their maintainer line by RFLP and RAPD analyses. Distinct polymorphisms were identified among the mtDNAs of cms-K, cms-V and their maintainer line, the orgnization and structure of mitochondrial genes such as atp9, atpA, coxII and cob were different remarkably among the three lines, which means that mtDNAs may be responsible for the formation of cms in wheat. However, it is difficult to identify which mtDNA fragment is definitely responsible for cms because there are so many differences between the mtDNAs of cms line and its maintainer line.

Key words: Wheat;Cytoplasmic male sterility (cms);Mitochondrial DNA(mtDNA);RFLP;RAPD

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引用本文：

李传友, 王斌. 小麦K型及V型细胞质雄性不育系线粒体DNA的比较分析[J]. 生理学报 1998; 50 (2): .

Li Chuanyou, Wang Bin. Mitochondrial DNAs of cytoplsamic male sterile lines of K- and V-type in wheat. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).