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未成熟油菜种子的子叶和花瓣细胞中BL的分布

何宇炯, 徐如涓

中科院上海植物生理研究所.上海 100032

摘要

以未成熟油菜种子的子叶和花瓣为材料,采用葡萄球菌A蛋白与胶体金连接的复合物为探针的免疫定位技术,研究和比较了两种不同组织细胞内BL的分布。

关键词： 油菜; 子叶; 花瓣; BL; 胶体金

BL localization in cotyledon of immature seed and petal cells of Brassica campestris

He Yujiong, Xu Rujuan

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

BL localization in cotyledon of immature seed and petal cells of Brassica campestris L. were studied using immunogold electron microscopy. The results showed that, in cells of cotyledon of immature seed, much more gold particles were localized in nuclei and cytoplasm, but no gold particles were found in vacuoles and cell walls. In petal cells gold particles were found mainly in nuclei, and only few in the cytoplasm.

Key words: Brassica campestris;Cotyledon petal;BL;Immunogold ;

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引用本文：

何宇炯, 徐如涓. 未成熟油菜种子的子叶和花瓣细胞中BL的分布[J]. 生理学报 1998; 50 (2): .

He Yujiong, Xu Rujuan. BL localization in cotyledon of immature seed and petal cells of Brassica campestris. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).