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转异源 adp基因烟草的叶外植体不同培养中的形态发生

王凌健, 倪迪安, 许智宏, 夏镇澳

中科院上海植物生理研究所.上海 200032

摘要

以转正义和反义 adp基因[生长素结合蛋白(ABP)的基因]烟草及对照的叶外植体为材料,在附加2mg/L或1mg/L的6-BA和不同浓度的NAA的MS培养基上进行不定芽分化试验。在较低NAA浓度条件下,转正义 adp基因烟草(SE2)的叶外植体分化的不定芽数高于对照(SR1)和转反义 adp基因烟草(AS3);而在较高NAA浓度条件下,AS3分化的不定芽数大于SE2和SR1。在培养基中加入生长素极性运输抑制剂HFCA后,不定芽分化受到抑制。

关键词： 烟草; 不定芽分化; 生长素结合蛋白; 生长素极性运输抑制剂

Studies on the morphogenesis in different cultures of leaf explants of sense and antisense adp transgenic tobacco

Wang Lingjian, Ni Di^an, Xu Zhihong, Xia Zhen^ao

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The leaf explants of tobacco (Nicotiana tobacum, SR1) and transgenic tobacco lines expressing foreign auxin binding protein (ABP) gene in sense (SE2) and antisense (AS3) orientation were cultured on MS media with 6-BA 2mg/L or 1mg/L and different concentrations of NAA for bud formation. At lower concentrations of NAA, more adventitious buds were formed in SE2 than that in SR1 and AS3, and the sensitivity of SE2 to auxin was increased. Feweer buds were formed in SE2 and SR1 than in AS3 at high concentrations of NAA, and the sensitivity of AS3 to auxin was decreased. When the leaf explants were cultured on media with polar transport inhibitor of auxin (HFCA), the differentiation of adventitious buds was inhibited.

Key words: Tobacco;Adventitious bud formation;Auxin binding protein (ABP);Auxin polar transport inhibitor(HFCA)

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引用本文：

王凌健, 倪迪安, 许智宏, 夏镇澳. 转异源 adp基因烟草的叶外植体不同培养中的形态发生[J]. 生理学报 1998; 50 (2): .

Wang Lingjian, Ni Di^an, Xu Zhihong, Xia Zhen^ao. Studies on the morphogenesis in different cultures of leaf explants of sense and antisense adp transgenic tobacco. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).