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缓激肽抑制大鼠DRG分离神经元的GABA激活电流

摘要

应用全细胞膜片箝技术在新鲜分离的大鼠背根神经节(DRG)神经元上研究缓激肽(BK)对#gamma#-氨基丁酸(GABA)反应的调制作用。结果发现:在34个对GABA反应的细胞中有31个细胞对BK敏感。在对BK敏感并引起内向电流的27个细胞中预加BK,对GABA-激活电流具有明显的抑制作用,如10~(-6)mol/L的BK可抑制GABA(10~(-4)mol/L)激活电流30%。BK可将GABA量效曲线明显下移,并抑制其最大反应达1/3以上,而不改变其K_(d)值。

关键词： 缓激肽; γ－氨基丁酸A受体; 背根神经节; 全细胞膜片箝

Inhibition of GABA-activated currents in isolated rat DRG neurons by bradykinin

Abstract

In the present paper, the modulation of GABA-activated currents by bradykinin (BK) was studied in DRG neurons freshly isolated from rat using whole-cell patch-clamp techniques. Among the neurons (n=34) responding to GABA, 27 were sensitive to BK. Pre-application of BK, eliciting an inward current alone, inhibited GABA-activated current markedly. For example, 10~(-6)mol/L BK suppressed GABA-activated current by about 30%. BK shifted the GABA dose-response curve downward obviously and depressed the maximal response to GABA by 1/3 or more while the K_(d) value was unchanged. The results suggest that BK inhibits GABA-activated current noncompetitively in rat DRG neurons.

Key words: Bradykinin;GABA_(A) receptor;Dorsal root ganglion;Whole-cell patch-clamp techniques

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引用本文：

. 缓激肽抑制大鼠DRG分离神经元的GABA激活电流[J]. 生理学报 1998; 50 (4): .

. Inhibition of GABA-activated currents in isolated rat DRG neurons by bradykinin. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).