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L-苯丙氨酸与血管平滑肌细胞增殖

摘要

用氚标胸腺嘧啶核苷掺入DNA合成法测定自发性高血压大鼠(SHR)与正常对照鼠的培养主动脉血管平滑肌细胞(VSMC)增殖,观察L-苯丙氨酸对细胞增殖、细胞生长及原癌基因 c-fos、c-myc表达的影响。结果显示:L-苯丙氨酸具有直接、特异性的抗VSMC增殖作用,提示L-苯丙氨酸在SHR大鼠中的抗高血压作用可能与血管平滑肌细胞增殖受抑有关。

关键词： L-苯丙氨酸; 血管平滑肌细胞; 细胞增殖

L-phenylalanine and smooth muscle cell proliferation from SHR and WKY rats

Abstract

Cell growth and proliferation were evaluated in cultured vascular smooth muscle cells (VSMCs) isolated from spontaneously hypertensive rat (SHR) and normotensive Wistar-Kyoto (WKY) rat aortae by measuring [~(3)]-thymidine incorporation into newly synthesized DNA and by determining cell number, respectively. The resutls showed that L-phenylalanine could exert a direct and specific antiproliferative effect on VSMCs suggesting that such effect can account for the antihypertensive action of this amino acid observed in SHR.

Key words: L-phenylalanine;Vascular smooth muscle cells;Cell growth

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引用本文：

. L-苯丙氨酸与血管平滑肌细胞增殖[J]. 生理学报 1998; 50 (4): .

. L-phenylalanine and smooth muscle cell proliferation from SHR and WKY rats. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).