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6-BA延缓大豆叶片衰老的作用与膜蛋白磷酸化状态的关系

摘要

关键词： 大豆叶片; 衰老; 6－BA; 蛋白激酶; 蛋白磷酸酯酶; 质膜蛋白磷酸化／脱磷酸化

The relationship between the effect of exogenous 6-BA in retarding soybean leaf senescence and plasma membrane protein phosphorylation

Abstract

The plasma membrane fraction isolated from primary leaves of 14 d soybean shoot showed protein kinase (PK) and protein phosphatase (PP) activities, and these activities declined dramatically when the leaves underwent senescence-inducing treatment. 6-BA pretreatment, which showed retarding effect on soybean leaf senescence, could sustain the activities of both PKs and PPs during the senescence-inducing treatment. The results of autoradiography suggested that the main PKs whose activities declined during leaf senescence were calcium-dependent protein kinases. The potent and specific inhibitor of protein phosphatase 1 and 2A, okadaic acid, could fasten leaf senescence.

Key words: Soybean leaf;Senesoence;6-BA;Protein kinase;Protein phosphatase;Plasma membran Protein phosphorylation/dephosphorylation

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引用本文：

. 6-BA延缓大豆叶片衰老的作用与膜蛋白磷酸化状态的关系[J]. 生理学报 1998; 50 (3): .

. The relationship between the effect of exogenous 6-BA in retarding soybean leaf senescence and plasma membrane protein phosphorylation. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).