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稻胚发育过程中细胞核内DNA增殖的特点

唐锡华, 张银屏

中科院上海植物生理研究所.上海 200032

摘要

应用显微分光光度技术测定的稻胚发育过程中核DNA含量的结果表明,球形胚中细胞核DNA含量较高,多倍性明显,C值较集中在6-12C,个别的最高达238或439C,它们可能为胚柄细胞;DNA含量差异明显可能与它们的分化潜能有关。分化初期、中期及成熟胚细胞核DNA含量一般不及球形胚高,不同倍性的频率分布亦较球形胚分散,但多倍性仍明显;胚分化完全后胚细胞核DNA含量较多变动在20-56C范围内,但盾状体细胞核DNA含量明显高于胚芽、胚根原基。

关键词： 稻; 胚胎发育; 核DNA含量; 多倍性; 差异复制

Peculiarities of nuclear DNA multiplication during embryogenesis in rice embryo cells

Tang Xihua, Zhang Yinping

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Nuclear DNA content was determined for glubular, differentiating and naturing embryos during rice embryogenesis. Measurment of nulear-DNA was done microspectro-photometrically using late prophase nuclei in Feulgen-DNA stained embryo squashes. The results show that differences in nuclear DNA cotent between embryos at various developmental stages. The nuclei measured in the globular embryo showed DNA values varying from 6 C to 12 C, i.e., they were polyploid. The highest DNA values found in the embryo were 238 C or 439 C. These cells may be located in the s u s pensor of early developing embryos. At later developmental stages of rice embryo, the nuclear DNA values varied from 20 C to 56 C. But the cells in the scutellum had higher C values than those in plumule and in primary radicle. These results of nuclear-DNA measuments for nuclei in glubular embryo as well as in different primordia in differentiated embryos offer strong evidence for differential DNA replication during rice embryogenesis.

Key words: Rice;Embryogenesis;Nuclear-DNA content;Polyploidy;Differential DNA replication

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引用本文：

唐锡华, 张银屏. 稻胚发育过程中细胞核内DNA增殖的特点[J]. 生理学报 1998; 50 (3): .

Tang Xihua, Zhang Yinping. Peculiarities of nuclear DNA multiplication during embryogenesis in rice embryo cells. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).