ISSN 0371-0874, CN 31-1352/Q

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玉米离体活性精细胞质膜的凝集素荧光标记

徐恒平, 曹宗巽

北京大学生命科学学院.北京 100871

摘要

荧光显微镜观察发现,10%-35%的精细胞可由FITC-Con A、FITC-WAG、FICT-RCA I所标记。在相应的单糖抑制实验中,未观察到被标记的精细胞。亦未观察到被FITC-SBA、FITC-PHA-L和FITC-UEA I所标记的精细胞。结果表明,玉米精细胞质膜分布有甘露糖、葡萄糖、半乳糖及乙酰氨基葡萄糖等残基。

关键词: 玉米; 精细胞; 质膜; 荧光标记

Labeling the plasma membrane of isolated viable sperm cells of Zea mays L. by fluorophore-conjugated lectins

Xu Hengping, Cao Zongxun

College of Life Sciences,Peking University.Beijing 100871

Abstract

Key words: Zea mays L.;Sperm cell;Plasma membtane;FITC-lectin labeling

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引用本文:

徐恒平, 曹宗巽. 玉米离体活性精细胞质膜的凝集素荧光标记[J]. 生理学报 1997; 49 (4): .

Xu Hengping, Cao Zongxun. Labeling the plasma membrane of isolated viable sperm cells of Zea mays L. by fluorophore-conjugated lectins. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).