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影响谷子原生质体看护培养的一些因素

董晋江, 夏镇澳

中科院上海植物生理研究所.上海 200032

摘要

不同种植物愈伤组织做看护细胞对谷子原生质体培养植板率有不同的影响。用光头稗草愈伤组织对谷子胚性、非胚性或中间型愈伤组织的原生质体进行看护培养,以对胚性愈伤组织原生质体的效果最好。看护培养主要是作用于原生质体形成完整细胞后的生长发育。试验没有观察到明显的看护细胞数量效应。

关键词： 看护培养; 胚性愈伤组织; 谷子; 棒头草; 光头稗草; 原生质体培养; 植板率

Effects of some factors of nurse culture on foxtail millet (Setaria italica(L.) Beauv) protoplasts

Dong Jinjiang, Xia Zhenao

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032

Abstract

Some aspects of nurse cultures by virtue of the protoplast culture of foxtail millet were studied. The results showed: the plating efficiencies of the protoplast cultures were influenced by the species of nurse cells. Using the calli of E. crusgalli as nurse cells, the nurse effect is better in the culture of embryogenic protoplasts than in the nonembryogenic or intermediate type protoplasts of foxtail millet. The effect of nurse cultures was the results of interactions between intact nursed cells and nurse cells, with the least influence on the protoplast growth; no significant quantity effects of nurse cells using in the experiments were detected.

Key words: Nurse culture;Embryogenic callus;Foxtail millet (Setaria italica);Polypogon fugax;Echinochloa crusgalli;Protoplast culture;Plating efficiency

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引用本文：

董晋江, 夏镇澳. 影响谷子原生质体看护培养的一些因素[J]. 生理学报 1997; 49 (4): .

Dong Jinjiang, Xia Zhenao. Effects of some factors of nurse culture on foxtail millet (Setaria italica(L.) Beauv) protoplasts. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).