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钙与钙调素对柑橘原生质体抗冻性的影响

李卫, 孙中海, 章文才, 马湘涛, 刘道宏

湖北大学生命科学系.湖北,武汉 430062；华中农业大学园艺系.湖北,武汉 430070

摘要

钙与钙调素对柑橘原生质体低温锻炼过程中抗冻力的获得有影响。加钙螯合剂或钙调素(CaM)拮抗剂TFP,能明显抑制柑橘原生质体抗冻性的表达。钙离子载体A_(23187)可提高未经低温锻炼的原生质体抗冻力,但该作用在钙调素拮抗剂TFP参入下则减弱,表明原生质体抗冻力的表达受钙和钙调素的调节。

关键词： 原生质体; 抗冻性; 钙; 钙调素; 柑橘

Role of Ca~(2+) and calmodulin on freezing tolerance of citrus protoplasts

Li Wei, Sun Zhonghai, Zhang Wencai, Ma Xiangtao, Liu Daohong

Department of Life Science,Hubei University.Wuhan 430062,Hubei；China

Abstract

Ca~(2+) and CaM plays important role in the acquistition of freezing tolerance by citrus protoplasts during cold acclimation. Ca~(2+) chelation or antagonism to calmodulin markedly inhibited the development of cold-induced freezing tolerance in protoplasts. Calcium ionophore A_(23187) strongly increased freezing tolerance of the protoplasts without cold acclimation, and the effects of A_(23187) was weakened by TFP, a calmodulin antagonist. These results suggest that freezing tolerance of protoplasts was mediated by Ca~(2+) and calmodulin.

Key words: Protoplast;Freezing-tolerance;Calcium;Calmodulin;Citrus

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引用本文：

李卫, 孙中海, 章文才, 马湘涛, 刘道宏. 钙与钙调素对柑橘原生质体抗冻性的影响[J]. 生理学报 1997; 49 (3): .

Li Wei, Sun Zhonghai, Zhang Wencai, Ma Xiangtao, Liu Daohong. Role of Ca~(2+) and calmodulin on freezing tolerance of citrus protoplasts. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).